A specific and sensitive radioimmunoassay for human choriogonadotropin.

Modification of the choriogonadotropin beta-subunit radioimmunoassay for determination of urinary choriogonadotropin.

The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial diluti...

DEVELOPMENT OF A RAPID AND SENSITIVE RADIOIMMUNOASSAY FOR MEASUREMENT OF AFLATOXIN B 1 IN BIOLOGICAL SAMPLES

Aflatoxin B I (AFB) isa well known hepatocarcinogen in several animal species and probably a causative agent in human hepatocellular carcinoma. Humans are exposed to AFB by ingesting contaminated food. Aflatoxin contamination encountered in human foods is usually at low levels which is difficult to measure by chromatographic methods. Therefore in the present study we have developed an immun...

A bedside urine test for human choriogonadotropin (hCG) as sensitive as serum radioimmunoassays.

Evaluation of five kits for detecting choriogonadotropin in urine.

We report an evaluation of the performance characteristics, accuracy, and sensitivity of five test kits designed to detect human choriogonadotropin (hCG) in urine: "Sensitex" (latex agglutination inhibition), "beta-Neocept" (hemagglutination inhibition), "Duoclone" (direct agglutination), "Tandem-Visual HCG" (enzyme immunoassay), and "Sensichrome" (enzyme immunoassay). For comparison, all speci...

Highly sensitive immunoenzymometric assay for human thyrotropin.

A sensitive assay procedure for immunoenzymometric assay of serum thyrotropin (TSH) was developed by making several modifications of the Enzymun-Test TSH kit (Boehringer, Mannheim GmbH). Serum samples were first incubated in plastic tubes precoated with monoclonal antibodies specific to the beta subunit of human TSH. After the tubes were washed, the TSH bound to the tubes was detected with pero...